Device

Part:BBa_K176230:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-13)


High input score automatic selector (with reporter): PoPS->GFP->K176229


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2270
    Illegal NheI site found at 2293
    Illegal NheI site found at 3119
    Illegal NheI site found at 3142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1401
    Illegal BglII site found at 2961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4586
    Illegal BsaI.rc site found at 662
    Illegal BsaI.rc site found at 4097


Design Notes

PoPS->GFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: Cell Density

Output3: GFP

Design:

Input1 PoPS generates Output3 GFP, LuxI-LVA and TetR-LVA.

pCon 0.15(K176008) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.


Source

K176106 K176226

References